Application Bibliography
| Application | Area Product | Reference | Date |
|---|---|---|---|
Fluorescence-detected photothermal infrared (FL-PTIR) hyperspectral imaging of autofluorescent biological materials and photosynthetic microorganisms. | Spectral Imaging SOLA Light Engine | 2024 | |
Imaging iGluSnFR3 glutamate sensor, jGCaMP8f calcium sensor and mRuby synapsin in rat hippocampal synaptic boutons. | Live Cell Microscopy SPECTRA Light Engine | 2024 | |
Six-color confocal phenotypic imaging of CRISPR–Cas9 knockouts in HUVEC cells | High-Content Screening SPECTRA Light Engine | 2024 | |
Fura-2 intracellular calcium imaging of DRG neurons | Live Cell Microscopy RETRA Light Engine | A γδ T cell-IL-3 axis controls allergic responses through sensory neurons. Nature (2024) 634:440–446 CH Flayer, IJ Kernin, CL Sokol et al. | 2024 |
Validation of aiSEGcell protocol applied to time-lapse imaging of NF-kappa B signaling dynamics | High-Content Screening SPECTRA X Light Engine | aiSEGcell: User-friendly deep learning-based segmentation of nuclei in transmitted light images. PLoS Comput Biol (2024) 20:e1012361 D Schirmacher, U Armagan, T Schroeder et al. | 2024 |
4-color confocal imaging of DAPI, ECFP, EGFP and mApple in mouse liver, lung, kidney, adipose and brain tissue sections using 405 nm, 446 nm, 477 nm and 545 nm CELESTA laser line outputs. | Confocal Microscopy CELESTA Light Engine | Visualizing the spatial organization of monocytes, interstitial macrophages, and tissue-specific macrophages in situ. Cell Rep (2024) 43:114847 M Petit, E Weber-Delacroix, A Boissonnas et al. | 2024 |
Freestanding bilayer microscopy (FBM) of human mechanosensitive potassium channel (hTRAAK) in lipid bilayer membranes | Biophysics SPECTRA X Light Engine | Freestanding bilayer microscope for single-molecule imaging of membrane proteins. Sci Adv (2024) 10:eado4722 G Pérez-Mitta, Y Sezgin, R MacKinnon et al., | 2024 |
Label-free optical redox imaging (ORI) for monitoring human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) differentiation and maturation | High-Content Screening SOLA Light Engine | 2024 | |
Imaging of membrane potential in rat hippocampal neurons using the genetically encoded fluorescent voltage indicator ASAP3β | Live Cell Microscopy SOLA Light Engine | 2024 | |
High-throughput in situ sequencing interrogation of the expression of 104 cell-type marker genes at cellular resolution in the mouse cerebral cortex. | Transcriptomics CELESTA Light Engine | 2024 | |
Total internal reflection fluorescence (TIRF) microscopy of Ca2+ dynamics and granule fusions in insulin-secreting INS-1 cells | Live Cell Microscopy SPECTRA Light Engine | 2024 | |
Iterative indirect immunofluorescence imaging (4i) of retinoblastoma (Rb) phosphorylation in MCF-10A human mammary epithelial cells | Immunofluorescence Microscopy SPECTRA X Light Engine | An intermediate Rb–E2F activity state safeguards proliferation commitment. Nature (2024) 631:424–431 Y Konagaya, D Rosenthal, T Meyer et al. | 2024 |
Multiplexed immunofluorescence imaging on large (average area = 0.25 mm2) sections of healthy, regenerating, and embolized human liver tissues | Immunofluorescence Microscopy CELESTA Light Engine | Cell atlas of the regenerating human liver after portal vein embolization. Nat Commun (2024) 15:5827 A Brazovskaja, T Gomes, B Treutlein et al. | 2024 |
Cryo-fluorescence microscopy detection of MX04-labeled amyloid in postmortem Alzheimer’s disease brain tissue | Pathology SOLA Light Engine | CryoET of β-amyloid and tau within postmortem Alzheimer’s disease brain. Nature (2024) 631:913–919 MAG Gilbert, N Fatima, RAW Frank et al. | 2024 |
Optical control of protein dimerization via reversible photoisomerization of an azobenzene ligand | Optogenetics SPECTRA X Light Engine | 2024 | |
Time-lapse confocal spinning disk imaging of heterotypic human ovarian fibroblast + serous ovarian cancer spheroid invasion of mesothelial cell monolayers | Confocal Microscopy CELESTA Light Engine | 2024 | |
Imaging T-cell calcium signaling elicited by a biomimetic antigen-presenting system (bAPS) | Bioengineering SPECTRA X Light Engine | 2024 | |
Confocal immunofluorescence localization of Aurora-A kinase (AurkA) and its major regulator TPX2 in hTERT RPE-1 cells | Confocal Microscopy CELESTA Light Engine | 2024 | |
Time-lapse imaging of periplasmic mTurquoise2 and cytoplasmic mCherry in Escherichia coli | Microbiology SOLA Light Engine | SlyB encapsulates outer membrane proteins in stress-induced lipid nanodomains. Nature (2024) 626:617–625 A Janssens, VS Nguyen, H Remaut et al. | 2024 |
Immunofluorescence identification of metastatic prostate cancer cells based on epithelial cell adhesion molecule (EpCAM) expression | Diagnostics SOLA Light Engine | A magnetic microneedle to isolate single immunomagnetically labeled cells. Lab Chip (2024) 24:460–466 M Stevens, P Harder, LWMM Terstappen | 2024 |
Imaging laminar flow of peripheral blood mononuclear cells (PBMC) and neutrophils during microfluidic acoustophoresis | Microfluidics CELESTA Light Engine | Label‐free separation of peripheral blood mononuclear cells from whole blood by gradient acoustic focusing. Sci Rep (2024) 14:8748 J Alsved, MR Charan, P Augustsson et al. | 2024 |
Imaging fission and fusion of neuronal mitochondria expressing photoactivatable GFP (paGFP) and mScarlet | Live Cell Microscopy AURA Light Engine | 2024 | |
In situ sequencing (ISS) for single-cell spatial expression profiling of the spatiotemporal cellular dynamics underlying autoimmune encephalomyelitis (EAE) evolution. | Transcriptomics SPECTRA X Light Engine | Cellular architecture of evolving neuroinflammatory lesions P Kukanja, CM Langseth, G Castelo-Branco et al. | 2024 |
Real-time measurement of polymer–nucleotide substrate (avidite) binding to DNA polonies | Genomic Analysis RETRA Light Engine | Sequencing by avidity enables high accuracy with low reagent consumption. Nat Biotechnol (2024) 42:132–138 S Arslan, FJ Garcia, M Previte et al. | 2024 |
Immunofluorescence detection of long myosin light-chain kinase (MLCK) splice variants in FFPE ileal tissue microarrays from Crohn’s disease patients. | Pathology SOLA Light Engine, AURA Light Engine | 2024 |